The Genome Engineering Core Facility generates mouse models for genetic diseases or genetic modifications that contribute to our understanding of (patho-)physiological mechanisms. We are able to generate knockout and knock-in models, reporter models and transgenic models. To do so, we utilise state-of-the-art technologies such as CRISPR/Cas-mediated genome engineering and numerous methods for the manipulation of murine zygotes, including microinjection, electroporation and AAV-mediated transduction.
For projects, we offer a comprehensive service covering strategy development, the administrative requirements for creating a genetically modified organism, the production, procurement and provision of all necessary materials, manipulation of zygotes and initial genotyping, right through to the establishment of a new line.
In addition, we offer in vitro fertilisation (IVF) or embryo transfer, as well as cryopreservation of sperm or embryos. The service is performed at the DZNE animal facility (Feodor-Lynen-Str. 17). However, animals may be delivered to the CAM of the BMC (without embryo transfer).
Related publications: Feiten et al., Nature Communications (2026), Asare et al., Immunity (2025), Kaji et al., Immunity (2024), Dunot et al., Neuron (2024) and Stafford et al., Nature (2022)
Contact
Dr. Tilman Breiderhoff
Division of Metabolic Biochemistry
Ludwig-Maximilians-Universität München
Stoffwechselbiochemie
Feodor-Lynen-Straße 17
81377 München